Part:BBa_K4438803
MN4_trigger_2_w/oPol
It is a single-stranded DNA sequence having 35 nucleotides. The 5’ and 3’ ends of the sequence have a few bases that are complementary to part Aptamer_MN4 (BBa_K4438800). The middle region has ssDNA sense T7 promoter sequence. Figure 1A) shows the secondary structure and minimum free energy.
Usage and Biology
This part can bind to Aptamer_MN4 (BBa_K4438800) due to complementarity and disrupt its three-way junction. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Quinine binds with cocaine-binding aptamer MN4 [1] and displaces the MN4_trigger_2_w/oPol(BBa_K4438803). This part has complete complementarity with MN4_Target_2(BBa_K4438804). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. All three parts can be used to detect different concentrations of quinine.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.
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